PSI SideView™ Imaging Microplate
Image System Optimization
Brightfield Imaging:
The illumination configuration in the figure below would improve brightfield imaging of the larvae by providing true transillumination of the sample. The illumination source can either be the standard blue LED illuminator under development for fluorescence excitation, or can be any other wavelength LED including white. Images collected using this approach are of the same quality as images collected using a standard microscope condenser.
Illumination apparatus for brightfield imaging of zebrafish larvae in SideView™ arrays. The LED illumination source is positioned adjacent to the well. Approximately 5% of the light is reflected from the top surface of prism through the well. The light that passes through the sample is efficiently reflected by prism toward the microscope objective.
